Sermorelin Acetate 5mg (Research Grade)

Product Identification & Core Specifications

• Chemical Name: Sermorelin Acetate

• Molecular Formula: C149​H246​N44​O42​S (as free base)

• Sequence: Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-NH2​

• Molecular Weight: $3357.9\text{ g/mol}$

• CAS Number: 86168-78-7

• Form: Lyophilized (Freeze-Dried) White Crystalline Powder

• Total Vial Content: $5\text{mg}$ ($\pm 0.05\text{mg}$ analytical variance)

• Purity Baseline: $\ge 99.0\%$ via High-Performance Liquid Chromatography (HPLC)

• Counter-Ion: Acetate

• Application Scope: In Vitro Cellular Signaling, Receptor Affinity Assays, Metabolic Modeling Studies

1. Structural and Chemical Overview

Sermorelin Acetate is a synthetically manufactured peptide comprised of 29 amino acid residues. It represents the truncated, fully functional amino-terminal fragment of the naturally occurring endogenous Growth Hormone-Releasing Hormone (GHRH1−29​). By isolating this specific sequence, molecular biochemists have stabilized the precise structural motif required to bind and activate human growth hormone-releasing hormone receptors ($\text{GHRH-R}$) in cellular laboratory models.

The synthesis process utilizes automated Solid-Phase Peptide Synthesis (SPPS) protocols, building the peptide chain from the C-terminus to the N-terminus. This meticulous layer-by-layer assembly ensures that the delicate steric configurations of the amino acid side chains are preserved. Following cleavage from the solid support resin, the crude peptide is subjected to intensive reverse-phase high-performance liquid chromatography ($\text{RP-HPLC}$) to isolate the target sequence from truncated synthesis byproducts or deletion sequences.

The resulting purified compound is combined with an acetate counter-ion to yield a highly stable, bioavailable crystalline salt. This salt configuration optimizes solubility profiles in aqueous laboratory solutions and helps preserve the structural topography of the peptide backbone during downstream experimental processes.

2. In Vitro Mechanisms of Action & Research Frameworks

In chemical modeling and tissue culture environments, Sermorelin Acetate mimics the dynamic actions of endogenous $\text{GHRH}$. Researchers utilizing this compound generally focus on several core pathways:

Receptor Kinetics and Ligand Affinity

Sermorelin Acetate functions as a selective agonist at the growth hormone-releasing hormone receptor ($\text{GHRH-R}$), a member of the secretin class of G-protein coupled receptors ($\text{GPCRs}$). Upon binding to the extracellular domain of the receptor in isolated cell cultures, the ligand induces a structural conformational shift that activates intracellular Gs​ alpha subunits. This cascade triggers the downstream up-regulation of adenylate cyclase, resulting in an acute increase in intracellular cyclic adenosine monophosphate ($\text{cAMP}$) concentrations.

Intracellular Signaling Pathways

The elevated presence of cellular $\text{cAMP}$ subsequently activates Protein Kinase A ($\text{PKA}$). This enzyme phosphorylates specific transcription factors—most notably the $\text{cAMP}$ response element-binding protein ($\text{CREB}$). In somatotropic cell assays, this transcriptional activation drives the up-regulation of genes responsible for growth hormone ($\text{GH}$) synthesis and cellular proliferation. Concurrently, the activation of $\text{PKA}$ opens voltage-dependent calcium channels, inducing an influx of extracellular Ca2+ that stimulates the exocytosis of pre-stored biochemical granules.

Negative Feedback Loop Mechanics

Sermorelin Acetate provides an essential baseline for studying homeostatic feedback control mechanics in vitro. Researchers utilize the compound to analyze how somatostatin (growth hormone-inhibiting hormone) interacts competitively at the receptor level to dampen $\text{cAMP}$ accumulation, offering insights into the complex regulatory balancing acts of endocrine signaling systems.

3. Analytical Validation and Quality Assurance Protocols

At Planet Peptide, scientific accuracy is our absolute priority. We recognize that subtle chemical anomalies or ambient degradation can introduce fatal confounding variables into your experimental data. For this reason, this $5\text{mg}$ lot of Sermorelin Acetate is subjected to a strict verification protocol:

Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC)

Every batch is assessed via $\text{RP-HPLC}$ utilizing a C18 column matrix under an optimized acetonitrile/water gradient containing trifluoroacetic acid ($\text{TFA}$) as a mobile phase modifier. The resulting chromatogram must display a singular, sharp peak indicating a chemical purity rating of $\ge 99.0\%$, ensuring the total absence of residual reagents, structural isomers, or truncated peptide contaminants.

Mass Spectrometry (MS) Analysis

To verify that the structural identity aligns with theoretical models, the purified compound is analyzed via Electrospray Ionization Mass Spectrometry (ESI-MS) or Matrix-Assisted Laser Desorption/Ionization ($\text{MALDI-TOF}$). The resulting mass spectra must accurately reflect the theoretical molecular weight of $3357.9\text{ g/mol}$ (free base format), confirming flawless amino acid sequencing and link matching.

Physical Conditioning via Lyophilization

Following chemical verification, the solution is portioned into pharmaceutical-grade borosilicate glass vials and subjected to deep freeze-drying (lyophilization). This process sublimates ambient water molecules directly from a frozen state, leaving behind a highly porous, stable crystalline cake. This step minimizes moisture content ($\le 5.0\%$), mitigating the risk of spontaneous hydrolysis during storage.

4. Laboratory Handling, Reconstitution, and Storage Guide

To maintain the structural integrity of the peptide bonds and maximize shelf-life reproducibility, lab technicians must adhere strictly to established handling workflows:

Long-Term and Short-Term Storage Requirements

• Lyophilized Form (Unopened): For maximum stability, store the dry crystalline vial in a dedicated laboratory freezer at -20°C or lower. Under these conditions, the peptide backbone remains secure against ambient degradation for up to 24 months.

• Short-Term Maintenance: Storage at standard refrigeration parameters (4°C) is acceptable for a duration not exceeding 12 months, provided the vial remains entirely unsealed and isolated from moisture.

• Protection from Environmental Catalysts: The peptide matrix must be kept away from direct ultraviolet ($\text{UV}$) light exposure and high-frequency thermal fluctuations.

Precise Reconstitution Workflows

1. Thermal Equilibration: Prior to introducing any liquid medium, allow the lyophilized vial to sit undisturbed until it reaches ambient room temperature ($20\text{°C}$ to $22\text{°C}$). Opening or injecting fluids into a cold vial can cause atmospheric moisture to condense rapidly inside the container, initiating immediate chemical degradation.

2. Medium Selection: Utilize sterile bacteriostatic water ($0.9\%$ benzyl alcohol) or a specialized laboratory buffer (such as sterile phosphate-buffered saline) depending on the requirements of your specific assay profile.

3. Fluid Introduction: Invert the vial at a 45-degree angle. Gently inject the solvent down the inner glass wall rather than directly onto the lyophilized cake to prevent mechanical shear stress on the peptide links.

4. Dissolution Management: Do not shake, vortex, or violently agitate the vial. Shaking will introduce air bubbles, cause frothing, and break down delicate peptide bonds. Instead, gently roll the vial between your palms or swirl it in a slow, circular motion until the crystalline cake has completely transitioned into a crystal-clear, uniform liquid matrix.

5. Post-Reconstitution Storage: Once transitioned into a liquid solution, the compound becomes significantly more susceptible to enzymatic and environmental degradation. Store the liquid solution at 4°C and utilize it within 21 days for maximum data accuracy.

5. Mandatory Legal Framework & Research-Use Limitations

LABORATORY RESEARCH USE ONLY

This product is synthesized, imported, and distributed strictly for in vitro laboratory experimentation, chemical modeling, and academic scientific evaluation.

• Not for Human Consumption: This compound is not a drug, cosmetic, food additive, or therapeutic item. It must never be administered via injection, oral ingestion, or topically to humans or animals under any circumstances.

• Regulatory Status: This material has not been evaluated, approved, or certified by the Food and Drug Administration (FDA) for the treatment, prevention, mitigation, or diagnosis of any medical condition.

• Buyer Responsibility: In purchasing this material, the buying institution or independent researcher assumes full legal and logistical liability for the safe storage, handling, containment, and disposal of the compound within an authorized research environment. Any usage that deviates from these terms constitutes a violation of our purchase agreements.